It is a glycoprotein containing 18% carbohydrate. This causes the horseradish peroxidase enzyme to break down into a second phase (HRP-II), ferric, and ferrous species. Purified HRP moved as a single band on Native PAGE with a molecular weight of 40Kda. pH Stability: pH 5.0 - 10.0 (25°C, 20 hrs.)

5 Scientists W. Anthony Oertling and Gerald T. Babcock attempted to mitigate this problem by lowering to cryogenic temperatures, pulsing a near-UV laser, and mixing hydrogen peroxide in the enzyme very quickly. Central European Journal of Chemistry 2009, 7 (3) , 423-428. Lab: Effect of Temperature on Peroxidase Background Data Procedure: Enzymes have an optimal temperature As the enzyme reaches its optimal temperature, the rate of the reaction increases If the enzyme becomes too hot, the enzyme starts to denature The optimal temperature of turnip Author information: (1)Voronezh State University, Russia. Thermal stability: Below 50°C (pH 6.0, 10 min.) Shaofen Guo, Qing Zhou, Tianhong Lu, Xiaolan Ding, Xiaohua Huang. Activity staining indicated that the purified protein was HRP.

[Effect of temperature on structure and functional properties of horseradish peroxidase]. The dynamics of structural and functional changes proceeding in a peroxidase molecule under the effect of temperature was studied. (Piracicaba, Braz.

Agric. Keywords Horseradish peroxidase, Concanavalin A agarose affinity chromatography, 4-Aminoantipyrine, Phenol, The pH optimum and temperature optimum was found out to be 6.0 and 40ºC respectively. DOI: 10.2478/s11532-009-0041-z. Peroxidase and polyphenoloxidase activities of litchi pericarp 213 Sci. Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. Horseradish peroxidase is a 44,173.9-dalton glycoprotein with 6 lysine residues which can be conjugated to a labeled molecule. APPLICATION: This highly purified preparation of horseradish peroxidase is useful as a protein tracer in histochemistry as well as cytochemistry, and also as a valuable experimental tool [Article in Russian] Artiukhov VG(1), Basharina OV, Iskusnykh AIu. Stabilization of horseradish peroxidase by covalent conjugation with dextran aldehyde against temperature and pH changes. Optimum temperature: 45 - 50°C.